Establishment and characterization of four new squamous cell carcinoma cell lines derived from oral tumors

Tatake, Revati J. ; Rajaram, Nirmala ; Damle, R. N. ; Balsara, Binaifer ; Bhisey, A. N. ; Gangal, Sudha G. (1990) Establishment and characterization of four new squamous cell carcinoma cell lines derived from oral tumors Journal of Cancer Research and Clinical Oncology, 116 (2). pp. 179-186. ISSN 0171-5216

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Four cell lines were established from squamous cell carcinomas (SCC) of the oral cavity. Cell lines AW 13516 and AW 8507 were derived from poorly differentiated SCC and epidermoid carcinoma of the tongue respectively. Cell line AW 10498 was derived from moderately differentiated SCC of the lower alveolus, and AW 9803 grew from a well-differentiated SCC of a retromolar trigone. The cultures showed typical epithelial cell morphology, numerous mitotic figures, occasional multinucleated giant cells, individual cell diskeratosis and nuclear and nucleolar abnormalities. The cell lines AW 13516 and AW 8507 were fast growers with a doubling time of 35.5 h and 31.9 h, respectively, which was independent of the initial seeding density. Cell lines AW 10498 (doubling time 52.2 h) and AW 9803 (doubling time 66 h) showed slower growth and had shorter doubling times at higher seeding densities. The presence of cytokeratins was detected in all the four cell lines by using polyclonal antikeratin antisera in indirect immunofluorescence and in Western blotting. None of the cell lines expressed major histocompatibility complex (MHC) class II antigens. MHC class I antigens were expressed by three cell lines but not by AW 9803. Flow cytometric analysis of DNA content and chromosomal studies suggested the presence of polyploidy and aneuploidy in all the four cell lines. Ultrastructural studies revealed typical epithelial cell features, such as the presence of desmosomes, tonofilaments and keratin bundles.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Squamous Cell Carcinoma; Epithelial Cell Lines; Keratin Expression
ID Code:10612
Deposited On:04 Nov 2010 06:00
Last Modified:01 Jun 2011 12:15

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