Analysis of DNA ploidy and expression of tumour-associated antigens on human oral carcinomas xenografted in nude mice

Damodaran, A. ; Gangal, S. G. ; Pichan, D. ; Amin, K. ; Fakih, A. R. ; Jambhekar, N. ; Rao, R. ; Chiplunkar, S. V. (1997) Analysis of DNA ploidy and expression of tumour-associated antigens on human oral carcinomas xenografted in nude mice Oral Oncology, 33 (6). pp. 402-407. ISSN 1368-8375

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Official URL: http://dx.doi.org/10.1016/S0964-1955(97)00040-7

Related URL: http://dx.doi.org/10.1016/S0964-1955(97)00040-7

Abstract

Human squamous cell carcinomas (SCC) of the oral cavity were successfully established as xenografts in nude mice. Tumours with higher malignancy scores and involvement of lymph nodes in patients were more readily accepted as xenografts in nude mice. The xenografted tumours were characterised with respect to morphology, histology, DNA index and expression of tumour-associated antigens (TAA). Flow cytometric analysis of cellular DNA content revealed that many of the xenografts retained the parent tumour DNA pattern while some of the xenografts showed progression to aneuploidy. All the xenografted tumours expressed TAA recognised by monoclonal antibody (MAb) 3F8E3. On Western blotting, MAb 3F8E3 recognised proteins of molecular weight 62-64 kDa on parent and xenografted tumours. In general, the xenografts reflect many of the characteristics of the tumours from which they were derived and may provide a useful model for investigating newer approaches of treatment and diagnosis.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Flow Cytometry; Oral Tumours; Athymic Mice; DNA Index; Mab Reactivity
ID Code:10604
Deposited On:04 Nov 2010 05:55
Last Modified:01 Jun 2011 12:14

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