New aqueous two phase system comprising polyethylene glycol and xanthan

Chethana, S. ; Rastogi, N. K. ; Raghavarao, K. S. M. S. (2006) New aqueous two phase system comprising polyethylene glycol and xanthan Biotechnology Letters, 28 (1). pp. 25-28. ISSN 0141-5492

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Official URL: http://link.springer.com/article/10.1007/s10529-00...

Related URL: http://dx.doi.org/10.1007/s10529-005-4682-5

Abstract

A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6–1.8% w/w; xanthan 0.24–0.28% w/w) while that of the top phase varied significantly (PEG 4–5% w/w, xanthan 0.05–1.37% w/w). Unlike conventional aqueous two phase systems, the viscosity of the top phase is also high and values are comparable to that of the bottom phase. When BSA was used as a model protein, it partitioned entirely into the bottom phase.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Aqueous Two Phase System; Partition Coefficient; Phase Diagram; Proteins; Xanthan
ID Code:105486
Deposited On:21 Dec 2017 11:49
Last Modified:21 Dec 2017 11:49

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