Reverse micellar extraction of β-galactosidase from barley (Hordeum vulgare)

Hemavathi, A. B. ; Umesh Hebbar, H. ; Raghavarao, K. S. M. S. (2008) Reverse micellar extraction of β-galactosidase from barley (Hordeum vulgare) Applied Biochemistry and Biotechnology, 151 (2). pp. 522-531. ISSN 0273-2289

Full text not available from this repository.

Official URL: http://link.springer.com/article/10.1007/s12010-00...

Related URL: http://dx.doi.org/10.1007/s12010-008-8228-x

Abstract

The reverse micellar system of sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/isooctane was used for the extraction and primary purification of β-galactosidase (EC 3.2.1.23) from the aqueous extract of barley (Hordeum vulgare) for the first time. The process parameters such as the concentration of the surfactant, the volume of the sample injected, and its protein concentration, pH, and ionic strength of the initial aqueous phase for forward extraction, buffer pH, and salt concentration for back extraction are varied to optimize the extraction efficiency. Studies carried out with both phase transfer and injection mode of reverse micellar extraction confirmed the injection mode to be more suitable for β-galactosidase extraction. The extent of reverse micellar solubilization of proteins increased with an increase in protein concentration of the feed sample. However, back extraction efficiency remained almost constant (13–14.4%), which indicates the selectivity of AOT reverse micelles for a particular protein under given experimental conditions. β-Galactosidase was extracted with an activity recovery of 98.74% and a degree of purification of 7.2-fold.

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Reverse Micellar System; Barley; β-Galactosidase; Phase Transfer; Injection Mode
ID Code:105458
Deposited On:21 Dec 2017 11:49
Last Modified:21 Dec 2017 11:49

Repository Staff Only: item control page