Roychoudhury, S. ; Shih, C. (1990) cis rescue of a mutated reverse transcriptase gene of human hepatitis B virus by creation of an internal ATG Journal of Virology, 64 (3). pp. 1063-1069. ISSN 0022-538X
|
PDF
- Other
1MB |
Official URL: http://jvi.asm.org/content/64/3/1063.short
Abstract
Using mutational analysis, we have investigated the translation strategy of the reverse transcriptase gene (pol) of human hepatitis B virus. It has been proposed that this pol gene product is synthesized as a core-pol fusion protein from a polycistronic mRNA template via ribosomal frameshifting, a mechanism often seen in retroelements. Our data indicate that creation of a novel ATG initiation codon near the original ATG can compensate for a lethal missense mutation at the first ATG position of the pol open reading frame. Genetic analysis has rigorously ruled out the possibilities of frameshifting, non-ATG initiation, or RNA editing. These results are discussed in the context of a 5'-end entry model versus a novel model of direct internal entry of ribosomes.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to American Society for Microbiology. |
ID Code: | 105298 |
Deposited On: | 21 Dec 2017 11:32 |
Last Modified: | 21 Dec 2017 11:32 |
Repository Staff Only: item control page