Aqueous two-phase extraction for downstream processing of enzymes/proteins

Raghavarao, K. S. M. S. ; Rastogi, N. K. ; Gowthaman, M. K. ; Karanth, N. G. (1995) Aqueous two-phase extraction for downstream processing of enzymes/proteins Advances in Applied Microbiology, 41 . pp. 97-171. ISSN 0065-2164

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This chapter discusses aqueous two-phase extraction (ATPE) for downstream processing of enzymes or proteins. Downstream processing in many fermentation processes accounts for a large share of the total cost. The ATPE appears to be a promising technique for efficient downstream processing and some successful applications of ATPE on large/industrial scale have been demonstrated. In order to develop effective downstream processing methods employing ATPSs, an interdisciplinary effort involving a combination of microbiological, biochemical, and engineering aspects is very essential. In addition, enough attention has been paid to microbiological and biochemical aspects. Mathematical modeling, which is highlighted in this chapter, is of immense use in predicting the protein enzyme partitioning without the measurement of an inordinately large number of parameters. Although, in principle, ATPE offers the advantage of easy adaptation of the extraction equipment used in the chemical or pharmaceutical industry to achieve efficient extraction, the drop dynamics and mass transfer aspects of this contacting equipment should be studied in detail employing various ATPSs and real systems involving the actual fermentation broths. Even if some of these aspects are addressed in greater depth by future researchers, the objective of this chapter can be considered fulfilled in view of the recognized scientific and industrial potential of ATPE.

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