Novel DNA sequence organization in rice genome

Gupta, Vidya S. ; Gadre, S. R. ; Ranjekar, P. K. (1981) Novel DNA sequence organization in rice genome Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 656 (2). pp. 147-154. ISSN 0005-2787

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DNA sequence organization in rice genome has been investigated by studying the extent of DNA reassociation as a function of DNA fragment size. The reassociation kinetics curves indicate that there is no sequence interspersion of repeated and single-copy sequences at a DNA length of 6500 nucleotide pairs. The reassociation at lower C0t values, however, shows that there is an interspersion of DNA sequences within the repetitive DNA fraction. The size distribution of the repeated sequences is determined by agarose gel electrophoresis of C0t 50 DNA fraction of 6500-nucleotide-pair-long rice DNA digested with S1 nuclease. About 35-40% of the S1-resistant duplexes consist of 6000-6400 nucleotide pairs and 60-65% have a fragment size less than 150 nucleotide pairs. Among plants, rice is the first genome in which no DNA sequence interspersion is observed.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:DNA Sequence Organization; Reassociation Kinetics; Genome Structure; (oryza Sativa)
ID Code:10394
Deposited On:04 Nov 2010 05:19
Last Modified:31 May 2011 11:37

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