Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

Hill, Joshua ; Beriwal, Shilpa ; Chandra, Ishwad ; Paul, Vinod K. ; Kapil, Aarti ; Singh, Tripti ; Wadowsky, Robert M. ; Singh, Vinita ; Goyal, Ankur ; Jahnukainen, Timo ; Johnson, James R. ; Tarr, Phillip I. ; Vats, Abhay (2008) Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli Journal of Clinical Microbiology, 46 (8). pp. 2800-2804. ISSN 0095-1137

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Official URL: http://jcm.asm.org/content/46/8/2800.full

Related URL: http://dx.doi.org/10.1128/JCM.00152-08

Abstract

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:103787
Deposited On:08 Dec 2017 09:26
Last Modified:08 Dec 2017 09:26

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