Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity

Bakshi, Rachit ; Mehta, Abhishek Kumar ; Sharma, Ritu ; Maiti, Souvik ; Pasha, Santosh ; Brahmachari, Vani (2006) Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity Biochemical and Biophysical Research Communications, 339 (1). pp. 313-320. ISSN 0006-291X

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.bbrc.2005.10.206

Abstract

The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. Here we report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity. A differential expression of the various domains within this gene is detected in human tissues while a ubiquitous expression is detected in mice. The intranuclear localization is demonstrated using antibodies directed against the DBINO domain of hINO80.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Ino80; SWI2/SNF2; Chromatin Remodeling; DNA Binding Domain; DNA Dependent ATPase
ID Code:103726
Deposited On:31 Mar 2017 07:23
Last Modified:31 Mar 2017 07:23

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