Role of G-quadruplex located at 5′ end of mRNAs

Agarwala, Prachi ; Pandey, Satyaprakash ; Maiti, Souvik (2014) Role of G-quadruplex located at 5′ end of mRNAs Biochimica et Biophysica Acta (BBA) - General Subjects, 1840 (12). pp. 3503-3510. ISSN 0304-4165

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Background: Secondary structures in 5′ UTR of mRNAs play a critical role in regulating protein synthesis. Though studies have indicated the role of secondary structure G-quadruplex in translational regulation, position-specific effect of G-quadruplex in naturally occurring mRNAs is still not understood. As a pre-initiation complex recognises 5′ cap of the mRNA and scans along the untranslated region (UTR) before initiating translation, the presence of G-quadruplex in 5′ region may have a significant contribution in regulating translation. Here, we investigate the role of G-quadruplex located at the 5′ end of an mRNA. Methods: Biophysical characterisation of putative G-quadruplexes was performed using UV and CD spectroscopy. Functional implication of G-quadruplex in the context of their location was assessed in cellulo using qRT-PCR and dual luciferase assay system. Results: PG4 sequences in 5′ UTR of AKT interacting protein (AKTIP), cathepsin B (CTSB) and forkhead box E3 (FOXE3) mRNAs form G-quadruplex whereas it is unable to form G-quadruplex in apolipoprotein A-I binding protein (APOA1BP). Our results demonstrated diverse roles of G-quadruplex located at 5′ end of mRNAs. Though G-quadruplex in AKTIP and CTSB mRNA act as inhibitory modules, it activates translation in FOXE3 mRNA. Conclusions: Our works suggests that G-quadruplex present at the 5′ terminal of an mRNA behaves differently in a different gene context. It can activate or inhibit gene expression.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:G-Quadruplex; UTR; AKTIP; CTSB; FOXE3; APOA1BP
ID Code:103642
Deposited On:04 Apr 2017 05:07
Last Modified:04 Apr 2017 05:07

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