Emergence of a unique recombinant form of HIV-1 from Manipur (India)

Sarkar, Roni ; Sarkar, Kamalesh ; Brajachand Singh, N. ; Manihar Singh, Y. ; Mitra, Debashis ; Chakrabarti, Sekhar (2012) Emergence of a unique recombinant form of HIV-1 from Manipur (India) Journal of Clinical Virology, 55 (3). pp. 274-277. ISSN 1386-6532

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jcv.2012.07.012

Abstract

Background: The AIDS epidemic in Manipur, India, manifests unique features, having co-circulation of B and C HIV-1 subtypes along with recombinant forms. Manipur has the highest incidence of HIV-1 infection compared to the other states of India, but limited information is available regarding the full-length sequence of HIV-1 recombinants. Objectives: To characterize the near full-length genome of a novel recombinant HIV-1 strain from an injecting drug user of Manipur. Study design: Viral RNA, extracted from the plasma of a male injecting drug user aged 35, was diagnosed with HIV-1 infection. Near full-length genome was amplified by polymerase chain reaction using primer walking approach. Phylogenetic relationships were determined with neighbor-joining trees. The recombination break points were detected using boot scan and Simplot analyses. Results: This recombinant predominantly had subtype C genome and exhibited mosaic structures with subtype B insertions at three different positions of HIV-1 genome. Simplot analysis of near full-length genome sequence from the recombinant HIV-1 strain, MAN86 exhibited similarity with the sequence of C.IN.93.93IN905 in its subtype C backbone, while the subtype B insertions showed resemblance with the sequence of B.TH.99.99TH_C1416. Conclusions: This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from India and Thailand.

Item Type:Article
Source:Copyright of this article belongs to Elsevier.
Keywords:Injecting Drug Users; Polymerase Chain Reaction; Human Immunodeficiency Virus; Near Full-Length Genome
ID Code:103310
Deposited On:05 Feb 2017 16:47
Last Modified:05 Feb 2017 16:47

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