Alternative splicing modulates subcellular localization of Laforin

Ganesh, Subramaniam ; Suzuki, Toshimitsu ; Yamakawa, Kazuhiro (2002) Alternative splicing modulates subcellular localization of Laforin Biochemical and Biophysical Research Communications, 291 (5). pp. 1134-1137. ISSN 0006-291X

Full text not available from this repository.

Official URL:

Related URL:


Laforin is a dual-specificity phosphatase coded by the EPM2A gene defective in Lafora's progressive myoclonus epilepsy. We reported earlier that laforin is a cytoplasmic protein associated primarily with polyribosome. In the present study we characterized the expression of an EPM2A splice variant, named C-terISO, originating from the usage of a novel exon located in the 3′-untranslated region of exon 4 and encoding a laforin isoform containing unique sequences at its carboxyl terminus. Transfection studies demonstrate that, in addition to cytoplasm, the protein coded by C-terISO was targeted to the nucleus, a distinctive feature that was not observed for laforin coded by the major transcript of the EPM2A gene. The unique C-terminal sequence did not affect laforin's affinity for polysome, but sequestered nearly an equal amount of the protein into the nucleus. Our results are significant in light of the finding that laforin is an active phosphatase; therefore, isoforms targeted to different cellular compartments might dephosphorylate and regulate distinct cellular substrates.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Epilepsy; EPM2A; Lafora Disease; Phosphatase; Cloning; Differential Splicing
ID Code:102823
Deposited On:01 Feb 2017 17:12
Last Modified:01 Feb 2017 17:12

Repository Staff Only: item control page