Basu, Snehasish ; Ghosh, Abhrajyoti ; Bera, Amit ; Saha, Manabendra N. ; Chattopadhyay, Dhrubajyoti ; Chakrabarti, Krishanu (2008) Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1 Bioresource Technology, 99 (17). pp. 8088-8094. ISSN 0960-8524
Full text not available from this repository.
Official URL: http://linkinghub.elsevier.com/retrieve/pii/S09608...
Related URL: http://dx.doi.org/10.1016/j.biortech.2008.03.032
Abstract
An extracellular pectate lyase (EC 4.2.2.2) was purified from the culture filtrate of a newly isolated Bacillus pumilus DKS1 grown in pectin containing medium. Using ion-exchange and gel filtration chromatography, this enzyme was purified and found to have a molecular weight of around 35 kDa. The purified enzyme exhibited maximal activity at a temperature of 75°C and pH 8.5. The presence of 1 mM calcium and manganese enhanced pectate lyase activity and was strongly inhibited by zinc, nickel and EDTA. The thermal inactivation studies revealed an entropy-enthalpy compensation pattern below a critical temperature. The alkaliphilicity and high thermostability of this pectate lyase may have potential implications in fibre degumming.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Bacillus pumilus DKS1; Pectate Lyase; Thermodynamic Activation and Inactivation Parameters |
| ID Code: | 10258 |
| Deposited On: | 04 Nov 2010 06:46 |
| Last Modified: | 29 Jan 2011 08:40 |
Repository Staff Only: item control page
