Infergen stimulated macrophages restrict Mycobacterium tuberculosis growth by autophagy and release of nitric oxide

Pahari, Susanta ; Khan, Nargis ; Aqdas, Mohammad ; Negi, Shikha ; Kaur, Jagdeep ; Agrewala, Javed N. (2016) Infergen stimulated macrophages restrict Mycobacterium tuberculosis growth by autophagy and release of nitric oxide Scientific Reports, 6 . Article ID 39492, 15 pages. ISSN 2045-2322

[img]
Preview
PDF - Other
1MB

Official URL: http://www.nature.com/articles/srep39492

Related URL: http://dx.doi.org/10.1038/srep39492

Abstract

IFN alfacon-1 (Infergen) is a synthetic form of Interferon (IFN)-α2b. Infergen has immunomodulatory activity and is effective against hepatitis C virus. However, the effect of Infergen (IFG) on Mycobacterium tuberculosis (Mtb) has not yet been reported. Therefore, for the first time, we have studied the influence of IFG in constraining the survival of Mtb in human macrophages. We observed that IFG significantly enhanced the maturation and activation of macrophages. Further, it substantially augmented the secretion of IL-6, Nitric Oxide (NO) and antigen uptake. Moreover, macrophages exhibited remarkably higher bactericidal activity, as evidenced by reduction in the Mtb growth. Infergen-mediated mechanism was different from the type-1 interferons; since it worked through the activation of NF-κB, phosphorylation of STAT-3 and Akt-PI3K that improved the bactericidal activity through autophagy and NO release. In future, IFG immunotherapy can be a novel strategy for treating patients and controlling TB.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
ID Code:102540
Deposited On:10 Jan 2017 12:11
Last Modified:10 Jan 2017 12:11

Repository Staff Only: item control page