Endocytosis by random initiation and stabilization of clathrin-coated pits

Ehrlich, Marcelo ; Boll, Werner ; van Oijen, Antoine ; Hariharan, Ramesh ; Chandran, Kartik ; Nibert, Max L. ; Kirchhausen, Tomas (2004) Endocytosis by random initiation and stabilization of clathrin-coated pits Cell, 118 (5). pp. 591-605. ISSN 0092-8674

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.cell.2004.08.017


Clathrin-coated vesicles carry traffic from the plasma membrane to endosomes. We report here the real-time visualization of cargo sorting and endocytosis by clathrin-coated pits in living cells. We have detected the formation of coats by monitoring incorporation of fluorescently tagged clathrin or its adaptor AP-2; we have also followed clathrin-mediated uptake of transferrin and of single LDL or reovirus particles. The intensity of a cargo-loaded clathrin cluster grows steadily during its lifetime and the time required to complete assembly is proportional to the size of the cargo particle. These results are consistent with a nucleation-growth mechanism and an approximately constant growth rate. There are no strongly preferred nucleation sites. A proportion of the nucleation events are weak and short lived. Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture.

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ID Code:102340
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