Antileishmanial efficacy of amphotericin B bearing emulsomes against experimental visceral leishmaniasis

Gupta, Swati ; Dube, Anuradha ; Vyas, Suresh P. (2007) Antileishmanial efficacy of amphotericin B bearing emulsomes against experimental visceral leishmaniasis Journal of Drug Targeting, 15 (6). pp. 437-444. ISSN 1061-186X

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Official URL: http://www.tandfonline.com/doi/full/10.1080/106118...

Related URL: http://dx.doi.org/10.1080/10611860701453836

Abstract

Amphotericin B (AmB) was formulated in trilaurin-based emulsomes (nanosize lipid particles) stabilized by soya phosphatidylcholine (PC), as a new delivery system for macrophage targeting for the treatment of visceral leishmaniasis (VL). Emulsomes were modified by coating them with macrophage-specific ligand (O-palmitoyl mannan, OPM). The antileishmanial activity of AmB-deoxycholate (AmB-Doc) and emulsome entrapped AmB was tested in vitro in Leishmania donovani infected macrophage-amastigote system (J774A.1 cells), which showed higher efficacy of OPM grafted AmB emulsomes (TLEs-OPM) over plain AmB emulsomes (TLEs) and AmB-Doc. The in vivo antileishmanial activity of the AmB (0.5 mg/kg) was tested in AmB-Doc, TLEs and TLEs-OPM forms against VL in L. donovani infected hamsters. Formulation TLEs-OPM eliminated intracellular amastigotes of L. donovani within splenic macrophages more efficiently (73.7 ± 6.7% parasite inhibition) than the formulation TLEs (51.7 ± 5.4% parasite inhibition) (P < 0.01) or AmB-Doc (30.4 ± 4.8% parasite inhibition) (P < 0.001). Our results suggest that these newer formulations (plain and ligand appended emulsomes) are a promising alternative to the conventional AmB-Doc formulation for the treatment of VL.

Item Type:Article
Source:Copyright of this article belongs to Informa Healthcare.
Keywords:Visceral Leishmaniasis; Macrophage Targeting; Emulsomes; Amphotericin B; O-Palmitoyl Mannan
ID Code:101796
Deposited On:11 Mar 2017 15:18
Last Modified:11 Mar 2017 15:18

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