Caerulomycin A suppresses immunity by inhibiting T cell activity

Singla, Arvind K. ; Gurram, Rama Krishna ; Chauhan, Arun ; Khatri, Neeraj ; Vohra, Rakesh M. ; Jolly, Ravinder S. ; Agrewala, Javed N. (2014) Caerulomycin A suppresses immunity by inhibiting T cell activity PLoS ONE, 9 (10). Article ID e107051-10 pages. ISSN 1932-6203

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Background: Caerulomycin A (CaeA) is a known antifungal and antibiotic agent. Further, CaeA is reported to induce the expansion of regulatory T cell and prolongs the survival of skin allografts in mouse model of transplantation. In the current study, CaeA was purified and characterized from a novel species of actinomycetes, Actinoalloteichus spitiensis. The CaeA was identified for its novel immunosuppressive property by inhibiting in vitro and in vivo function of T cells. Methods: Isolation, purification and characterization of CaeA were performed using High Performance Flash Chromatography (HPFC), NMR and mass spectrometry techniques. In vitro and in vivo T cell studies were conducted in mice using flowcytometry, ELISA and thymidine-[methyl-3H] incorporation. Results: CaeA significantly suppressed T cell activation and IFN-γ secretion. Further, it inhibited the T cells function at G1 phase of cell cycle. No apoptosis was noticed by CaeA at a concentration responsible for inducing T cell retardation. Furthermore, the change in the function of B cells but not macrophages was observed. The CaeA as well exhibited substantial inhibitory activity in vivo. Conclusion: This study describes for the first time novel in vitro and in vivo immunosuppressive function of CaeA on T cells and B cells. CaeA has enough potential to act as a future immunosuppressive drug.

Item Type:Article
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ID Code:101692
Deposited On:12 Feb 2018 12:54
Last Modified:12 Feb 2018 12:54

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