Regulation of proteasome activity in activated human platelets

Nayak, Manasa K. ; Kumar, Kailash ; Dash, Debabrata (2011) Regulation of proteasome activity in activated human platelets Cell Calcium, 49 (4). pp. 226-232. ISSN 0143-4160

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Ubiquitin-proteasome system has emerged a central player in regulation of diverse cellular processes. However, relevance of proteasome activity in platelets, which are terminally differentiated enucleate cells, is not clear. In this report we show that activation of platelets with physiological agonists was associated with 7–10 -fold rise in proteasomal activity. Elevation of cytosolic calcium with A23187 or thapsigargin resulted in significant increase in enzymatic activity, while treatment with intracellular calcium chelator or inhibitor of inositol trisphosphate receptor attenuated proteasomal enzymes in collagen-stimulated platelets. Specific inhibitors of protein kinase C as well as calpain, too, downregulated proteasome function. To conclude, proteasomal enzymatic activity in platelets is regulated by cytosolic calcium through Ca2+-dependent downstream effectors like calpain and protein kinase C.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Platelet Activation; Intracellular Calcium; Proteasome; Calpain
ID Code:101251
Deposited On:04 Feb 2017 17:20
Last Modified:04 Feb 2017 17:20

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