Regulation of β-catenin stabilization in human platelets

Kumari, Sharda ; Dash, Debabrata (2013) Regulation of β-catenin stabilization in human platelets Biochimie, 95 (6). pp. 1252-1257. ISSN 0300-9084

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The Wnt/β-catenin pathway controls developmental processes and homeostasis; however, abnormal activation of this pathway has been linked to several human diseases. Recent reports have demonstrated regulation of platelet function by canonical and non-canonical Wnt signalling. Platelet aggregation plays a crucial role in haemostasis and thrombosis. Here we report for the first time that, induction of sustained aggregation of platelets by a strong agonist in the presence of calcium was associated with nearly complete proteolysis of β-catenin, which was abrogated upon depletion of calcium from platelet suspension. β-catenin cleavage was disallowed in absence of aggregation, thus implicating integrin αIIbβ3 engagement in β-catenin proteolysis. Degradation of β-catenin was blocked partially by inhibitors of either proteasome or calpain and completely when cells were exposed to both the inhibitors. Protein kinase C inhibition, too, abolished β-catenin degradation. Thus activities of proteasome, calpain and protein kinase C regulate stabilization of β-catenin in aggregated human platelets.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Platelet Aggregation; Wnt Signalling; Protein Kinase C; Proteasome; Calpain; Thrombin
ID Code:101016
Deposited On:04 Feb 2017 17:20
Last Modified:04 Feb 2017 17:20

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