Induction of heterozygous and homozygous diploid gynogenesis in Betta splendens (Regan) using hydrostatic pressure

Kavumpurath, Soosamma ; Pandian, T. J. (1994) Induction of heterozygous and homozygous diploid gynogenesis in Betta splendens (Regan) using hydrostatic pressure Aquaculture Research, 25 (2). pp. 133-142. ISSN 1355-557X

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...

Related URL: http://dx.doi.org/10.1111/j.1365-2109.1994.tb00569.x

Abstract

Optimum conditions for hydrostatic pressure treatment for duplication of chromosome set in gynogenetically activated fighting fish, Betta splendens (Regan), eggs were identified. Maximum survival of heterozygous gynogens was 50%, when 2.5-min-old eggs, after insemination with UV irradiated tilapia sperm, were pressure shocked at 7000psi for 6 min. The frequency (21%) of homozygous gynogenetic fry was high, when the 34min (post-insemination) old eggs, which were inseminated with tilapia sperm, were pressure shocked for 5 min. Sex ratio of gynogenetic progeny suggested that the mechanism of sex determination in this fish is homogametic female and heterogametic male.

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