Bayer, Philipp E. ; Ruperao, Pradeep ; Mason, Annaliese S. ; Stiller, Jiri ; Chan, Chon-Kit Kenneth ; Hayashi, Satomi ; Long, Yan ; Meng, Jinling ; Sutton, Tim ; Visendi, Paul ; Varshney, Rajeev K. ; Batley, Jacqueline ; Edwards, David (2015) High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus Theoretical and Applied Genetics (TAG), 128 (6). pp. 1039-1047. ISSN 0040-5752
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Official URL: http://doi.org/10.1007/s00122-015-2488-y
Related URL: http://dx.doi.org/10.1007/s00122-015-2488-y
Abstract
The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Nature Switzerland AG. |
| ID Code: | 124991 |
| Deposited On: | 23 Dec 2021 07:52 |
| Last Modified: | 23 Dec 2021 07:52 |
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