Direct imaging of pH1N1 2009 influenza virus replication in alveolar pneumocytes in fatal cases by transmission electron microscopy

Basu, Atanu ; Shelke, Vijay ; Chadha, Mandeep ; Kadam, Dilip ; Sangle, Shashi ; Gangodkar, Shobha ; Mishra, A. C. (2011) Direct imaging of pH1N1 2009 influenza virus replication in alveolar pneumocytes in fatal cases by transmission electron microscopy Journal of Electron Microscopy, 60 (1). pp. 89-93. ISSN 0022-0744

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Official URL: http://jmicro.oxfordjournals.org/content/60/1/89.s...

Related URL: http://dx.doi.org/10.1093/jmicro/dfq081

Abstract

Human influenza virus pandemics constitute a major global public health issue. Although studies on autopsy specimens from the recent pandemic by the 2009 influenza A (H1N1) virus have revealed a broad spectrum of pathologic findings, direct electron microscopic studies of the lung tissue from influenza fatalities are few. In this study, we examined five well-preserved pulmonary necropsy specimens from fatal cases of laboratory-confirmed pH1N1 from India. The novel observations in comparison with earlier reports included direct imaging of influenza virus budding within dilated cisternae of pneumocytes, cell-free virus emerging from the cell membrane of a pneumocyte in the alveolar lumen, presence of polymorphonuclear cells with red blood cells as inflammatory exudates close to hyaline membranes and extensive cytoplasmic degeneration of epithelial cells of the alveolar lining. These observations are in consistent with the earlier findings and emphasize the possible role of this virus directly infecting cells of the lower respiratory tract as a key event in the rapid pathogenesis of pH1N1 disease process.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Pandemic Influenza Virus 2009; Electron Microscopy; Necropsy; Lung
ID Code:73626
Deposited On:06 Dec 2011 11:07
Last Modified:06 Dec 2011 11:07

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