Absence of simian virus 40 in human brain tumors from Northern India

Engels, Eric A. ; Sarkar, Chitra ; Daniel, Richard W. ; Gravitt, Patti E. ; Verma, Kusum ; Quezado, Martha ; Shah, Keerti V. (2002) Absence of simian virus 40 in human brain tumors from Northern India International Journal of Cancer, 101 (4). pp. 348-352. ISSN 0020-7136

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/ijc.106...

Related URL: http://dx.doi.org/10.1002/ijc.10621

Abstract

Simian virus 40 (SV40), a monkey polyomavirus, was a contaminant of early poliovirus vaccines administered to millions of individuals in the 1950s and early 1960s. SV40 causes brain tumors in laboratory animals, and SV40 DNA sequences have been variably identified in human choroid plexus tumors and ependymomas. We studied the possible association between SV40 and human brain tumors in northern India, where humans have frequent contact with SV40-infected rhesus macaques. DNA from pathologic specimens from 33 ependymomas, 14 choroid plexus tumors and 18 control brain tissues (contused brain, brain metastases) was extracted and analyzed under masked conditions. We used real-time PCR to detect and quantify SV40 (T antigen) and human (GAPDH) DNA sequences. The SV40 PCR assay detected as few as 10 copies of SV40 DNA and had a linear range from 1 × 102 to 1 × 106 copies. SV40 DNA was detected in 1 specimen (an ependymoma). However, few SV40 DNA copies were detected in this sample (<10 copies, equivalent to <1 copy/350 cells, based on simultaneous GAPDH quantification), and SV40 was not detected when this sample was retested. Our findings do not support a role for SV40 in choroid plexus tumors or ependymomas from northern India.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Simian Virus 40; Brain Tumor; India; Ependymoma; Choroid Plexus Tumor
ID Code:56366
Deposited On:23 Aug 2011 11:31
Last Modified:23 Aug 2011 11:31

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