Shetty, Gunapala ; Krishnamurthy, Hanumanthappa ; Krishnamurthy, Hegganahalli N. ; Bhatnagar, Ajay S. ; Moudgal, Nuggehalli R. (1998) Effect of long-term treatment with aromatase inhibitor on testicular function of adult male bonnet monkeys (M. radiata) Steroids, 63 (7-8). pp. 414-420. ISSN 0039-128X
![]()
|
PDF
- Publisher Version
192kB |
Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00391...
Related URL: http://dx.doi.org/10.1016/S0039-128X(98)00042-7
Abstract
The role/need for estrogen in regulating testicular function of adult male bonnet monkeys (M. radiata) has been investigated by dosing orally a group of five normal males 2.5 mgs of CGP 47645, a long-acting nonsteroidal aromatase inhibitor (AI), once every 5 days for over 150 days. Such treatment resulted in a 10-fold increment in nocturnal serum testosterone (T) levels, which were sustained for 85 days of treatment, and a twofold increment in basal serum T levels was present throughout the 150 days of treatment. Analysis of ejaculated semen showed a marked reduction (~90%) in sperm counts in four out of five monkeys between Days 55-85 of treatment. During this period, the motility score also was markedly reduced from a normal score of 3-5 to 0-2. Flow cytometric analysis of testicular germ cells obtained from biopsy tissue taken on Days 63 and 120 indicated a marked reduction only in elongating/elongated spermatid population (compared to Day 0 values), suggesting inhibition in spermiogenic process. Epididymal sperm maturation also seemed effected as sperm chromatin, on flow cytometric analysis for decondensability following exposure to 5 mM dithiotreitol, showed to be in a hypercondensed state. This study thus indicates that estrogen has an important role in providing normal testicular and sperm function in the primate.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Estrogen; Primate; Spermatogenesis; Testicular Function |
ID Code: | 21560 |
Deposited On: | 22 Nov 2010 06:46 |
Last Modified: | 17 May 2016 05:45 |
Repository Staff Only: item control page