Creating a macromolecular receptor by affinity imprinting

Vaidy, A. A. ; Lele, B. S. ; Kulkarni, M. G. ; Mashelkar, R. A. (2001) Creating a macromolecular receptor by affinity imprinting Journal of Applied Polymer Science, 81 (5). pp. 1075-1083. ISSN 0021-8995

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/app.152...

Related URL: http://dx.doi.org/10.1002/app.1529

Abstract

A molecularly imprinted polymeric receptor for trypsin was synthesized by employing a novel technique that is a combination of affinity separation and molecular imprinting. An enzyme-inhibitor complex of trypsin and N-acryloyl para-aminobenzamidine was polymerized with acrylamide and N,N'-methylene bis-acrylamide. Template trypsin was extracted out to obtain an affinity-imprinted polymer. Control experiments were performed to demonstrate the synergistic affinity-imprinting effect. The percentage of crosslinker used was the crucial factor in determining the imprinting efficacy of the polymers. Imprinted polymer containing 50% crosslinker exhibited a linear Scatchard plot. Unlike non-imprinted gel, the receptor exhibited almost exclusive recognition of trypsin in an individual batch experiment as well as from a mixture of trypsin and chymotrypsin.

Item Type:Article
Source:Copyright of this article belongs to John Wiley & Sons, Inc.
Keywords:Affinity; Imprinting; Trypsin Receptor
ID Code:17068
Deposited On:16 Nov 2010 08:35
Last Modified:04 Jun 2011 08:12

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