Mukhopadhyay, Arka ; Dasgupta, Anjan Kumar ; Chattopadhyay, Dhrubajyoti ; Chakrabarti, Krishanu (2012) Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles Bioresource Technology, 116 . pp. 348-354. ISSN 09608524
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Official URL: http://doi.org/10.1016/j.biortech.2012.03.094
Related URL: http://dx.doi.org/10.1016/j.biortech.2012.03.094
Abstract
The activity and half-life of pectate lyase (PL) from Bacillus megaterium were nine- and 60-fold, respectively, higher at 90 °C in the presence of hydroxyapatite nanoparticles (NP-PLs) than in the presence of 1 mM CaCl2. Thermodynamic analysis of the nanoparticle-induced stability revealed an enhanced entropy–enthalpy compensation by the NP-PLs since a reciprocal linearity of the enthalpy–entropy change to 90 °C was observed. Without nanoparticles, the linearity range was 70 °C. Such compensation reflected the maintenance of the native structure of proteins. The remarkable enhancement of activity and stability of the NP-PL system at high temperatures may be utilized commercially e.g. in the food industry or the processing of natural fibers that may require a thermotolerant enzyme.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Ltd |
| ID Code: | 132489 |
| Deposited On: | 19 Dec 2022 05:11 |
| Last Modified: | 19 Dec 2022 05:11 |
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