PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence

Singh, Parul ; Rao, Rameshwaram Nagender ; Reddy, Jala Ram Chandra ; Prasad, R. B. N ; Kotturu, Sandeep Kumar ; Ghosh, Sudip ; Mukhopadhyay, Sangita (2016) PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence Scientific Reports, 6 . Article ID 21624. ISSN 2045-2322

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Official URL: http://www.nature.com/articles/srep21624

Related URL: http://dx.doi.org/10.1038/srep21624

Abstract

The role of the unique proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family of proteins in the pathophysiology and virulence of Mycobacterium tuberculosis is not clearly understood. One of the PE family proteins, PE11 (LipX or Rv1169c), specific to pathogenic mycobacteria is found to be over-expressed during infection of macrophages and in active TB patients. In this study, we report that M. smegmatis expressing PE11 (Msmeg-PE11) exhibited altered colony morphology and cell wall lipid composition leading to a marked increase in resistance against various environmental stressors and antibiotics. The cell envelope of Msmeg-PE11 also had greater amount of glycolipids and polar lipids. Msmeg-PE11 was found to have better survival rate in infected macrophages. Mice infected with Msmeg-PE11 had higher bacterial load, showed exacerbated organ pathology and mortality. The liver and lung of Msmeg-PE11-infected mice also had higher levels of IL-10, IL-4 and TNF-α cytokines, indicating a potential role of this protein in mycobacterial virulence.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
ID Code:103550
Deposited On:09 Mar 2018 10:52
Last Modified:09 Mar 2018 10:52

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