Kamthan, Ayushi ; Kamthan, Mohan ; Chakraborty, Niranjan ; Chakraborty, Subhra ; Datta, Asis (2012) A simple protocol for extraction, derivatization, and analysis of tomato leaf and fruit lipophilic metabolites using GC-MS Protocol Exchange . No pp. given. ISSN 2043-0116
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Official URL: http://www.nature.com/protocolexchange/protocols/2...
Related URL: http://dx.doi.org/10.1038/protex.2012.061
Abstract
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
Item Type: | Article |
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Source: | Copyright of this article belongs to Nature Publishing Group. |
Keywords: | Gas Chromatography-Mass Spectrometry; Metabolomics; Lipophilic |
ID Code: | 100385 |
Deposited On: | 12 Feb 2018 12:29 |
Last Modified: | 30 Jan 2023 06:00 |
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